Screening of the specific biomarkers from serum of the patient suffered from ovarian cancer by SELDI TOF mass spectrometry
- VernacularTitle:卵巢癌患者血清比较蛋白质组学研究
- Author:
Rong WEI
;
Chunfang GAO
;
Guan ZHAO
- Publication Type:Journal Article
- Keywords:
ovarian neoplasms;
tumor markers, biological;
surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI TOF MS)
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To approach the feasibility of identifying the specific biomarker of ovarian cancer by SELDI TOF mass spectrometry. Methods The relative contents of serum protein of both 24 the patients with ovarian cancer and 56 cases of healthy people were tested by IMAC3 chip and proteinchip reader (CipherGen Inc., VS). Results On the M/Z values ranged from 4000Da to 10000Da, there were six kinds of protein contents in the serum of the were obviously different between the two groups. Among them the serum protein of M/Z value 4472Da may be regarded as a specific biomarker of ovarian cancer. In the learning mode, all the 24 patients and 56 control people were diagnosed and distinguished out correctly, while in the test mode, 23 patients were correctly diagnosed and 56 control people were distinguished out, the total accuracy was 98 75%(79/80), and the sensitivity and specificity were 95 8%(23/24) and 100%(56/56), respectively. Conclusion Ovarian cancer can be quickly and correctly diagnosed by this method with high sensitivity and specificity. That will be widely used in clinical application
