Experimental study of a fibronectin like peptide P19
- VernacularTitle:纤维连接蛋白模拟肽P19的实验研究
- Author:
Jianghai NING
;
Hongchen LIU
;
Huixi WANG
- Publication Type:Journal Article
- Keywords:
fibronectins;
hydroxyapatites;
peptides;
osteoblasts
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective A fibronectin like peptide was designed and synthesized based on the structure and function relationship study of extra cellular matrix protein in order to search after a kind of new method to improve the bioactivity of hydroxyapatite based biomaterials. Methods A peptides named P19 with two functional groups was synthesized by employing solid phase peptide synthesis. The synthesized peptides were preliminarily purified with Sephadex G 15 column chromatography and further modified with air oxidation method. The purity of P19 were determined with RP HPLC. The molecular weight of peptide was identified with TOF MS. Its binding characteristics and bioactivity was investigated. Results P19 has high affinity with hydroxyapatite and cannot be eluted under physiological condition. Its dissociation constant is 14 3?0 8?mol/L and the maximum of attached peptides by 100?g HA is 2 57?0 51nmol. It can affect the in vitro calcification of gel system. The plate pretreated with P19 can enhance the number of attached cells and the proliferation of attached cells is also promoted. The fibronectin like activity was also proved by the competitive inhibition effect of P19 on the cell attachment. It also can promote the proliferation of UMR 106 cell line. Conclusion The synthesized peptide possess two function as design, it may have extensive application prospect as an ingredients of HA based materials or coating layer of implant
