Cloning and analysis of HSPC016 gene in dermal papilla cells
- VernacularTitle:毛乳头细胞HSPC016基因亚型全长cDNA的克隆及功能分析
- Author:
Jiwen WANG
;
Zhiqiang SONG
;
Yongjia DENG
- Publication Type:Journal Article
- Keywords:
dermal papilla cells;
genes, HSPC016;
transcriptional regulation
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone the full length cDNA sequence of HSPC016 gene, an aggregative growth related gene in dermal papilla cells (DPC). Analyze its characteristics and predict its biological function in the phase of growth and differentiation for DPC. Methods Rapid amplification of cDNA ends (RACE) technology was used for full length cDNA amplification. Bioinformtic methods were used to analyze the chromosome location, protein sequence, domain and possible biological function of the gene. Results Two isoforms of HSPC016 gene were obtained from DPC. They were 400bp and 493bp, respectively. The gene was mapped on chromosome 3 q21 31, and was conservative on evolution. HSPC016 protein had 64aa, belonged to PD053992 protein family; its functional domain was homologous to T2FA gene. Conclusion HSPC016 was a transcriptional modulatory gene. Its protein product may act as a subunit of a transcriptional complex and play a role on DPC growth and differentiation through modulating other genes' transcription within nucleus
