Preparation and characterization of two monoclonal antibodies against human VSIG4
- VernacularTitle:两株鼠抗人VSIG4单克隆抗体的研制及其生物学特性的研究
- Author:
Shijie ZHANG
;
Lei WANG
;
Jiamin WANG
;
Xi CHEN
;
Jing JIANG
;
Yueying WANG
;
Yumin HU
;
Xueguang ZHANG
;
Zongjiang GU
- Publication Type:Journal Article
- Keywords:
VSIG4;
Hybridoma;
Monoclonal antibody;
Macrophage
- From:
Chinese Journal of Immunology
2010;26(1):66-69,78
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare anti-VSIG4 monoclonal antibodies and characterize their biological functions.Methods: BALB/c mice were immunized with transfected cell line (L929/VSIG4L) as immunogen.The spleen B cells of the mice were fused with SP2/0 and hybridoma cells were screened with transfected cell line (L929/VSIG4) by FCM.After acquisition of the hybridomas secreting anti-VSIG4 mAb,their biological activities were investigated by indirect immunofluorescence,Western blot,competitive inhibition test,and MTT assay.Results:Two stable hybridomas,9A7 and 9D5 were obtained,which could continuously secrete specific anti-VSIG4 monoclonal antibodies.The following biological activity studies showed that these monoclonal antibodies could recognize the natural VSIG4 expressed on the macrophages and several cancer cell lines,such as Jurkat,THP-1 and H446.Furthermore,they could block the inhibitory effects of VSIG4 on proliferation of T cells in vitro.Conclusion: Two hybridomas secreting anti-VSIG4 monoclonal antibodies have been established.These monoclonal antibodies provide useful tools for further studying VSIG4's biological function and its unknown receptor.
