GPCR-G? fusion protein and its applications on ligand screening of orphan G protein-coupled receptors
- VernacularTitle:GPCR-G?融合蛋白及其在oGPCRs配基筛选中的应用
- Author:
Fangming WU
;
Yongxu LIU
- Publication Type:Journal Article
- Keywords:
orphan G protein coupled receptors;
G protein;
fusion protein;
ligand screening
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
GPCR-G alpha fusion protein has been used to investig ate receptors in recent years. The expression of GPCR-G alpha fusion protein en sured defined 1∶1 stoichiometry, the physical proximity of GPCR to G alpha subu nit, and was suitable for high-throughput ligand screening. The fusion protein technology provided a novel strategy to study orphan GPCRs. Obviously, it will p lay important roles in ligand screening of the orphan GPCRs and exert its signif icances in the R&D of novel drugs targeted at oGPCRs.
