Effects of inhibitors of glycosphingolipid biosynthesis D-PDMP on proliferation and differentiation of neural stem cells.
- VernacularTitle:神经鞘糖脂生物合成抑制剂D-PDMP对神经干细胞增殖、分化能力的影响
- Author:
Ling LIN
;
Jianyin LIN
;
Zhihong ZHENG
;
Qiong JIANG
;
Jianshi HU
- Publication Type:Journal Article
- Keywords:
neural stem cell;
D-PDMP;
proliferation;
differentiation
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of inhibitors of glycosphingolipid biosynthesis D-PDMP on the proliferation and differentiation of neural stem cells. Methods Neural stem cells cultured in vitro were treated with different concentration of D-PDMP. The effects of D-PDMP on proliferation and differentiation of neural stem cells were evaluated by counting of neurospheres size and cellular number, MTT assaying and the experiment of inducing differentiation.Results Neurospheres were smaller in size after treated with D-PDMP. The data of cellular counting and MTT assay suggested that D-PDMP could inhibit proliferation of neural stem cells and lead cells death in higher concentration, D-PDMP also reduced the differentiation ability of neural stem cells. Although differentiated cells were fewer, they could be induced into neurons and astrocytes.Conclusion The inhibitors of glycosphingolipid biosynthesis D-PDMP could inhibit the proliferation of neural stem cells, even lead cells death. It also reduced the differentiation ability of neural stem cells.
