The effect of Shuanghuanglian on the load of hepatic HCMV DNA in mice infected with HCMV
- VernacularTitle:双黄连对人巨细胞病毒感染小鼠肝组织病毒DNA载量影响
- Author:
Jianjun WU
;
Guiyu LIU
;
Guihai CHEN
;
Shu HOU
;
Caohui FANG
;
Mingli WANG
- Publication Type:Journal Article
- Keywords:
human cytomegalovirus(HCMV);
Shuanghuanlian;
quantitive;
real-time PCR
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of Shuanghuanglian on the the load of hepatic HCMV DNA in mice infected with HCMV.Method The model of mice hepatitis infected with HCMV was prepared. 10 days after treated with Shuanghuanlian,the hepatic HCMV DNA was detected qualitatively by polymerase chain reaction;the level of alanine aminotransferase (ALT) and aspartate aminotransferase(AST) in serum were assayed by spectrophotometer;and hepatic pathology was observed with HE staining.Results HCMV DNA load in infected model group was 3875.4760 copies/mg,while in high and middling dosage shuanghuanglian therapy group it was 8.3261 copies/mg、9.1476 copies/mg respectively;It decreased significantly compared with the former(P0.05);low dosage of shuanghuanglian(HCMV DNA load was 3812.8980 copies/mg) had no notable difference compared with infected model group;serum transaminase (ALT,AST) levels significantly elevated in infected model group; serum transaminase level decreased sharply and liver histological pathology was lighten after treated with high and middling dosage of Shuanghuanglian(P
