HTS model for protein tyrosine kinase inhibitors
- VernacularTitle:酪氨酸蛋白激酶抑制剂的高通量筛选模型
- Author:
Guangliang HAN
;
Nianyong SHANG
;
Guanhua DU
- Publication Type:Journal Article
- Keywords:
PTK;
inhibitor;
model;
HTS
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To introduce a high throughput screen model for PTK inhibitors. Methods With tyrosine containing polypeptides as substrate, in reaction buffer PTK extracts catalyzed the phosphorylation of tyrosine residues. ELISA method was used to measure the activity of protein tyrosine kinase. Resutls The IC_ 50 of genistein was 110 ?mol?L -1. From more than 7000 samples, 16 were found to possess some degree of PTK inhibiting activity. Conclusion This HTS model for screening PTK inhibitors was stable and sensitive.
