Relationship between mast cells and renal interstitial fibrosis in interstitial nephritis
- VernacularTitle:肥大细胞与间质性肾炎间质纤维化的关系
- Author:
Zhao TAN
;
Xuefei TIAN
;
Hongrui DONG
;
Yipu CHEN
- Publication Type:Journal Article
- Keywords:
Nephritis,interstitial;
Fibrosis;
Mast cells;
Transforming growth factor-?1;
Proteinase-activated receptor 2
- From:
Chinese Journal of Nephrology
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential relationship between tryplase-positive mast cells (MCs) infiltration and renal interstitial fibrosis in acute interstitial nephritis (AIN) and chronic interstitial nephritis (CIN). Methods Renal biopsy specimens from patients with AIN (n=11) and CIN (n=16) were studied and 11 patients in minimal change diseases (MM)were as controls. Histochemistry and immunohistochemistry staining assay were applied to delect the expression of tryptase, proteinase-activated receptor-2 (PAR-2), TGF-?1 and collagen type I (Col I )in the renal tissues. Immunofluo-rescence double-staining assay was used to assess the relationship among MCs, PAR-2-positive cells, and TGF-?1-positive cells in the renal interstitium respectively. Results MCs in AIN and CIN were significantly increased compared with those in controls and were mainly scattered in the fibrotic areas of renal interstitium. The relative immunostaining areas for PAR-2, TGF-?1 in the renal tubular epithelial cells (RTECs) and Col I were significantly larger in AIN and CIN than those in controls respectively (P
