Protective effect of rosiglitazone on cyclosporine A-induced nephrotoxicity in rat NRK cells
- VernacularTitle:罗格列酮对环孢素A所致大鼠成纤维细胞肾毒性的保护作用
- Author:
Zhangsuo LIU
;
Genyang CHENG
;
Haijian LI
;
Shuzhen LIU
- Publication Type:Journal Article
- Keywords:
Cyclosporine A;
Fibroblast;
Peroxisomes;
Transforming growth factor ?1;
Rosiglitazone
- From:
Chinese Journal of Nephrology
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of rosiglitazone (RSG) on normal rat kidney cells (NRK) damaged by cyclosporine A(CsA). Methods The NRK cells were cultured with CsA or with CsA plus rosiglitazone. The cellular proliferation was determined by MTT colorimetry. The mRNA expression of TGF-?1 and PPAR? was detected by RT-RCR. Protein levels of PPAR?, p-ERK,FN and AT1R were examined by Western blotting. The level of TGF-?1 in the supernatants was measured by enzyme-linked immunosorbant assay (ELISA). Results CsA inhibited the proliferation of NRK cells in dose and time dependent manner (P
