Phospholipase C?1 and NF-?B are required for cell-matrix adhesion of colorectal cancer cells
- VernacularTitle:磷脂酶C?1及NF-?B在结直肠癌细胞与基质黏附中的作用及信号转导机制(英文)
- Author:
Xiumei LI
;
Xiaochun BAI
;
Fan DENG
;
Di LU
;
Shenqiu LUO
- Publication Type:Journal Article
- Keywords:
phospholipase C?1;
colorectal neoplasms;
adhesion;
epidermal growth factor;
NF-?B
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the function and mechanism of phospholipase C?1 (PLC?1) in cell-matrix adhesion in colorectal cancer. Methods: Highly metastatic colorectal cancer cell line LoVo and lowly metastatic colorectal cancer cell line SW480 were subjected to cell-matrix adhesion assay. U73122 (a specific inhibitor of PLC) and pyrrolidine dithiocarbamate (PDTC) (an inhibitor of NF-?B) were used to study the effect of PLC?1 and NF-?B on cell-matrix adhesion. Furthermore, Western blot and gel electrophoresis mobility shift assay (EMSA) were performed to detect the mechanism of PLC?1 in colorectal cancer cell adhesion to matrix. Results: Inhibition of PLC?1 or NF-?B resulted in reduction of cell-matrix adhesion in a dose-dependent manner in LoVo cells(P
