bcl-2 antisense oligodeoxynucleotide increases sensitivity of NCI-H460 cell lines to docetaxel and radiation
- VernacularTitle:bcl-2反义寡核苷酸增强NCI-H460细胞对紫杉特尔和放射线的敏感性
- Author:
Dongmei HE
;
Huan ZHANG
- Publication Type:Journal Article
- Keywords:
bcl-2;
antisense oligonucleotide;
NCI-H460 cells;
docetaxel;
radiation;
sensitivity
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine whether the bcl-2 antisense oligonucleotide (ASODN) can increase the sensitivity of NCI-H460 cell lines to docetaxel or radiation. Methods: Docetaxel in combination with bcl-2 ASODN or non-sense oligonucleotide (NSODN) was used to treat NCI-H460 cells, and then IC_~50 of docetaxel against NCI-H460 cells was determined with MTT method. In radiation group, NCI-H460 cells were divided into pure radiation, radiation plus bcl-2 ASODN, and radiation plus NSODN groups. The inhibition rates of cell growth were assayed by MTT, and the expression levels of Bcl-2 protein were assayed by immunofluorescence. Results: IC_~50 in bcl-2 ASODN plus docetaxel, pure docetaxel and NSODN plus docetaxel groups was (0.101?0.009) ?mol/L,(0.183?0.018)?mol/L, and (0.179?0.016)?mol/L, respectively. The differences between the first group and the latter 2 groups were significant (P
