Expression of human telomerase reverse transcriptase in urine exfoliative cells of patients with bladder transitional cell carcinoma
- VernacularTitle:尿路移行细胞癌尿脱落细胞人端粒酶逆转录蛋白的表达及其意义
- Author:
Li GAO
;
Qing YANG
;
Minghua ZHU
- Publication Type:Journal Article
- Keywords:
telomerase reverse transcriptase;
urine exfoliated cell;
transitional cell carcinoma;
immunocytochemistry
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of human telomerase reverse transcriptase (hTERT) in urine exfoliated cells of benign and malignant urothelial diseases, in an attempt to evaluate the role of detecting hTERT gene expression in cytologic diagnosis of transitional cell carcinoma. Methods: The expressions of hTERT gene were detected by immunocytochemistry staining in 54 patients with transitional cell carcinoma(25 cases with grade Ⅰ, 26 with grade Ⅱ and 13 with grade Ⅲ) and 20 controls, including 10 patients with urinary tract stone and 10 healthy volunteers. The urine exfoliative cells of both groups were also stained by H-E and the results were compared with those of immunocytochemistry.Results: hTERT was detected in 74.1% (40/54) of patients with transitional cell carcinoma and in 5%(1/20) of the controls (P
