Stability evaluation of thymosin ?_1 under microsphere preparation condition
- VernacularTitle:微球制剂条件下胸腺肽?_1的稳定性评价
- Author:
Yan ZHU
;
Dongfeng YIN
;
Jing LIU
;
Yanqiang ZHONG
- Publication Type:Journal Article
- Keywords:
Thymosin ?_1;
chromatography,high pressure liquid;
stability;
microspheres
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the stability of thymosin ?_1 microspheres under preparation conditions.Methods: Using HPLC method,we investigated the influence of different temperatures(-20 ℃, 6-8℃,60℃),sonification time(10-60 s) and pH values (pH 4.0,7.0 and 10.0) on thymosin ?_1 stability.Results: Under the conditions of frozen environment(-20℃) and refrigeration(6-8℃), thymosin ?_1 remained stable for 30 d.Thymosin ?_1 did not degrade in 60℃ water bath for 12 h or in phosphate buffer solution(PBS,pH 4.0) under 37℃ for 14 h.The sonification time within 60 s was found to be safe for the preparation.Conclusion: Thymosin ?_1 is stable under these preparation conditions.
