Amplification and identification of dendritic cells from mouse bone marrow
- VernacularTitle:小鼠骨髓来源的树突状细胞体外扩增与鉴定
- Author:
Junbo XIA
;
Kui WU
;
Kun SUN
;
Changzheng WANG
- Publication Type:Journal Article
- Keywords:
dendritic cell;
granulocyte macrophage colony stimulation factor;
purification
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the methods for in vitro generating dendritic cells (DCs) from mouse bone marrow and to identify it with morphological, phenotype determination. Methods Cells isolated from mice bone marrow were cultured in GM-CSF (20 ng/ml), differentiating into dendritic cells. Morphological changes were observed by optical phase contrast microscopy, and surface molecules including CD11 C, CD80, MHCⅡ were detected by FACS. Results A large number of typical DCs were observed after culturing for 10 d. FACS analysis showed that the amplified DCs could express CD11 C, CD80, MHCⅡ. Conclusion A large quantity of highly pure BM-DC can be obtained by this method.
