Protein kinase A signal pathway regulating corticotropin-releasing hormone mRNA expression in hypothalamic slice of rats in vitro
- VernacularTitle:PKA信号通路调控体外培养大鼠脑片的CRHmRNA表达的研究
- Author:
Lizhao CHEN
;
Minhui XU
;
Jihon ZHOU
- Publication Type:Journal Article
- Keywords:
stress;
hypothalamic slices in vitro;
corticotropin-releasing hormone mRNA;
corticotropin-releasing hormone receptor
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate protein kinase A (PKA) signal regulatory mechanism of corticotropin-releasing hormone (CRH) mRNA expression, after CRH stimulated neuron of hypothalamic slices in rats in vitro. Methods After CRH stimulated corticotropin-releasing hormone type 1 receptor (CRH1R) of hypothalamic slices in rats in vitro, the changes of activity of PKA signal pathway and their relationship with CRH mRNA expression were observed by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting. Results CRH may cause the remarkable increase in phosphorylated PKA (P-PKA), phosphorylated cyclic adenosine monophosphate response element-binding protein (P-CREB) and CRH mRNA content in hypothalamic slices in rats. However, CP-l54526 or H89 could have significant inhibition effects on the synthesis of P-PKA, P-CREB and CRH. Conclusion PKA signal pathway in ultrashort positive feedback control of CRH secretion in hypothalamus in the stress due to severe traumas.
