Study of changes of OPG and RANKL expression in denervated rabbits
- VernacularTitle:失神经支配兔下颌骨骨折愈合过程中CGRP对OPG/RANKL表达的影响
- Author:
Lin XU
;
Yinhui TAN
;
Jianhua WANG
;
Gang ZHANG
- Publication Type:Journal Article
- Keywords:
calcitonin gene-related peptide;
osteoprotegerin;
receptor activator nuclear factor kappa B ligand;
mandibular fracture
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of the expression of osteoprotegerin (OPG) and receptor activator nuclear factor kappa B ligand (RANKL) during the healing of mandibular fracture after the transection of the inferior alveolar nerve (IAN) in rabbits. Methods Forty rabbits were randomly divided into 2 groups and each group consisted of 20 animals. The rabbits of the first group underwent mandibular fracture and transection of IAN and those of the second group underwent mandibular fracture only. The rabbits were killed at 7, 14, 21, 28 day after fracture. The callus tissue of the rabbits was collected and paraffin sections of the callus tissue were prepared. The callus sections were subjected to HE staining. The effects of calcitonin gene-related peptide (CGRP) on the expression of OPG and RANKL were observed with immunohistochemical staining. Results There were only a few CGRP immunoreactive nerve fibers at day 7 after IAN section. Low level of OPG expression was found throughout the repair process but RANKL was intensively expressed in the rabbits of the first group. Conclusion CGRP can up-regulate the expression ratio of OPG/RANKL and promote the healing process of fracture. The loss of CGRP is unfavorable to bony healing.
