Nordihydroguaiaretic acid causes the ovarian functional disorders in rats
- VernacularTitle:去甲二氢愈创木酸对大鼠卵巢功能的影响
- Author:
Xiaohua WU
;
Lin WANG
;
Changxu SHI
- Publication Type:Journal Article
- Keywords:
angiogenesis inhibitor;
NDGA;
ovarian function
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the ovary in rats. Methods Forty female SD rats in normal estrous cycles were randomly divided into PBS group and NDGA group, subcutaneously injected of PBS solution and 3% NDGA at the dose of 30 mg/kg respectively every other day for 16 d. The serum concentrations of follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E 2) and progesterone (P) were measured by chemiluminoimmunoassay before and after administration and the mating was confirmed by the occurrence of vaginal plugs and the morphological changes of uterus and ovaries were examined by immunohistochemistry after administration. Results None of the NDGA-treated rats were able to successfully mate as the absence of vaginal plugs were detected. The endometrium could maturate in all of the NDGA-treated rats, but there had a decrease not only in the number of endometrial glands, but also the proliferation rate of the glandular epithelial cells, the endometrial stroma cells, the vessels and the microvacular density (P
