Expression of connexin 43 gene and the gap junction intercellular communication among cultured unstable detrusor cells
- VernacularTitle:不稳定逼尿肌Cx43基因表达及其与逼尿肌细胞间缝隙连接通讯功能的关系研究
- Author:
Jianli FENG
;
Bo SONG
- Publication Type:Journal Article
- Keywords:
detrusor instability;
connexin 43;
detrusor instability;
gap junction intercellular communication
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression changes of connexin 43 (Cx43) gene and the functional changes of the gap junction intercellular communication (GJIC) among cultured unstable detrusor cells and their roles in the development of detrusor instability (DI). Methods Forty healthy female Wistar rats were divided into two groups: DI group and normal control group. RT-PCR and Western blot techniques were employed to detect the expression of Cx 43 mRNA and protein in the cultured detrusor cells. Scrape loading dye transfer (SLDT) technique was used to monitor the GJIC among cultured detrusor cells. Results The expression of Cx43 mRNA and protein in DI group was much higher than that in normal control group (P
