Tissue-engineered bone constructed with demineralized bone matrix scaffold: an experimental study
- VernacularTitle:脱钙骨基质支架构建组织工程骨的实验研究
- Author:
Jie LIU
;
Jianzhong XU
;
Xuquan WANG
;
Fei LUO
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cells;
demineralized bone matrix;
tissue-engineered bone
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the tissue-engineered bone with the allogenic demineralized bone matrix (DBM) as the scaffold material and the in vitro cultured human mesenchymal stem cells (hMSCs) and to evaluate the geosteogenesis efficacy and safety by implantation of tissue-engineered bone in nude mice subcutaneously. Methods After cultured and amplified, hMSCs were combined with DBM at the density of 1.857?10 6/ml to construct the tissue-engineered bone. The hMSCs and DBM combination was cultured in vitro for 3, 5, 7 d and tested for biocompatibility under scanning electron microscope and each sample was implanted into 36 nude mice subcutaneously, DBM as the control. The culture suspension of the hMSCs and DBM combination was injected into another 4 nude mice to observe the oncogenesis. Specimens were harvested after 4, 8, 12 weeks for histological observation. Results At day 3 of culture, hMSCs and DBM combined well and at day 5, the cell matrix was productive and full of the scaffold. The osteocytes were found inside in the scaffold in the experimental groups, while no osteocytes in the control groups. Conclusion The DBM has good biocompatibility, providing good three-dimension space for hMSCs. It is safe and successful to construct the tissue-engineered bone by the method we introduced.
