Preparation and immunological activity in vitro of human prostatic carcinoma-targeted liposome microbubbles
- VernacularTitle:人前列腺癌靶向超声造影剂的制备及其体外实验研究
- Author:
Lisha ZHUO
;
Rui LI
;
Yanli GUO
;
Xing HUA
;
Yun HE
;
Zheng LIU
;
Chixu FU
- Publication Type:Journal Article
- Keywords:
liposome contrast agent;
prostate carcinoma;
polyclonal antibody
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare human prostatic carcinoma-targeted ultrasound contrast agent and assess its targeted ability in vitro. Methods Human prostatic carcinoma-specific polyclonal antibody PSM(C-15) was attached to the surface of self-made liposome microbubbles by electrostatic attraction to prepare targeted microbubbles. Immunofluorescent staining assay was used to identify the combination of PSM(C-15) with liposome microbubbles and the targeted microbubbles were added to prostatic-carcinoma cells and then observed under the light and fluorescence microscope to evaluate the targeting ability of the targeted liposome microbubbles with prostatic carcinoma cells in vitro, while the common liposome microbubbles were controls. Results Targeted microbubbles were positive in immunofluorescent straining assay. In vitro study of the targeting ability showed the targeted microbubbles could actively adhere to LNCaP cell. While the control was negative. Conclusion The targeted liposome contrast agent with highly specific biological activity was prepared successfully. The contrast agent could bind to human prostatic carcinoma cells specially and effectively in vitro.
