Testicular MCP-1 expression in mouse induced by systemic inflammation
- VernacularTitle:细菌脂多糖诱导单核细胞趋化蛋白-1在小鼠睾丸中表达
- Author:
Shaohong DING
;
Gang CHEN
;
Chenjuan YAO
;
Junkang JIANG
;
Chun WANG
;
Yutaka NAKAHORI
- Publication Type:Journal Article
- Keywords:
LPS;
MCP-1;
Systemic inflammation;
Testis
- From:
Chinese Journal of Immunology
2009;25(12):1105-1107,1111
- CountryChina
- Language:Chinese
-
Abstract:
Objective:There is a large of population of macrophages resident in the testicular interstitial tissue under normal conditions and they are increased during inflammation.The mechanisms involved are unclear.This study focused on the expression of monocyte chenoattractant protein-1 (MCP-1) in the mouse testis before and after an intraperitoneal injection of LPS.Methods:The expression of MCP-1 in testis was detected by using reverse transcription-polymerase chain reaction and Western blot,the immunofluorescent technique was used to detect the localization of MCP-1 protein in testis.Results:In the normal testis,the expression of MCP-1 mRNA and protein was detectable by RT-PCR and immunofluorescent technique,respectively.The level of testicular MCP-1 mRNA increased dramatically at 3-24 h after LPS treatment,the level of MCP-1 protein increased at 12 h after LPS treatment.The MCP-1 was localized in the testicular interstitial tissue.Conclusion:MCP-1 may play a role in maintaining the resident macrophage population in normal testis and regulating monocyte and macrophage influx in inflammatory testis.
