Construction of pIRES2-hBSP-EGFP vector and stably expression in MDA-MB-231BO breast cancer cells
- VernacularTitle:骨唾液蛋白非融合荧光蛋白载体的构建及其在特异性骨转移乳腺癌细胞中的稳定表达
- Author:
Hongyan DU
;
Jie WANG
;
Yong GUO
- Publication Type:Journal Article
- Keywords:
bone sialoprotein (BSP);
EGFP;
breast cancer cell;
transfection;
stably expression
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To constitute a higher expression system and to obtain the breast cancer cell strains in which BSP is stably expressed. Methods hBSP gene was subcloned from pB-hBSP vector by PCR. The PCR fragment was inserted into the eukaryon expression vector, pIRES2-EGFP, which allow exogenous protein and EGFP to express respectively. The recombinant vector, pIRES2-hBSP-EGFP, was transfected into human breast cancer cells with Lipofectamine TM 2000. The expression of symbol protein EGFP, could be conveniently observed with fluorescent microscope. Results The recombinant pIRES2-hBSP-EGFP plasmid was constituted and successfully transfected into breast cancer cells. In the breast cancer cell strain hBSP and EGFP were expressed. Conclusion The successful constitution and transfection of hBSP and EGFP nonfusion expression vector laid a foundation for the further study on the effect of BSP on breast cancer metastasizing to bone in vivo or in vitro.
