Establishment and Evaluation of a Rat Model of Adriamycin-Induced Myocardial Injury
- VernacularTitle:阿霉素心肌损伤大鼠模型的制备与评价
- Author:
Dan SU
;
Pengfei LIU
;
Liu ZHANG
;
Bogen SONG
;
Guifen ZHAO
- Publication Type:Journal Article
- Keywords:
Adriamycin;
Myocardial injury;
Rat model
- From:
Acta Laboratorium Animalis Scientia Sinica
2009;17(6):442-444,illust 2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate a rat model of adriamycin-induced myocardial injury.Methods Tweenty-four male SD rats were randomly divided into two groups. The adriamycin (ADR) group (n=15) was injected intraperitoneally with six equal doses of ADR (2 mg/kg each time, with a cumulative dose of 12 mg/kg) over a period of two weeks, while the control group (n=9) received an equivalent volume of normal saline intraperitoneally alone. The Rats were observed for 5 weeks after treatment. The general conditions and mortality were recorded. At the end of experiment, the parameters of hemodynamics including LVSP, LVEDP, ±LVdP/dtmax and HR were measured. The pathological changes were analyzed by histological hematoxylin-eosin staining. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activity as well as malondialdehyde (MDA) content in heart tissue were measured according to the kit instructions, respectively. Results The cumulative mortality in the ADR group was 40%. Compared with the control group, LVEDP and -LVdP/dtmax were significantly increased in the ADR group (P<0.001, P<0.05). The results of pathological examnation of the ADR group were consistent with characteristic changes of myocardial injury. Myocardial malondialdehyde (MDA) content was significantly increased and glutathione peroxidase (GSH-Px) activity decreased in the ADR group compared with those in the controls.Conclusion A rat model of adriamycin-induced myocardial injury has been successfully established by administration of ADR (cumulative dose, 12 mg/kg) to rats in six equal intraperitoneal injections over a period of 2 weeks, resulting in cardiac dysfunction and histological damages.
