Interaction of novel gene AngRem104 and Bardet-Biedl syndrome 2 protein in mammalian cells
- VernacularTitle:新基因AngRem104与Bardet-Biedl综合征2蛋白的相互作用
- Author:
Hong ZHANG
;
Yanling ZHANG
;
Ping HOU
;
Xiubin LIANG
;
Haiyan WANG
- Publication Type:Journal Article
- Keywords:
Yeasts;
Two hybrid system techniques;
Bardet-Biedl syndrome;
Immunoprecipitation;
AngRem104
- From:
Chinese Journal of Nephrology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen for proteins interacting with novel gene AngRem104 and to identify the putative interaction of novel gene AngRem104 and Bardet-Bied1 syndrome 2 (BBS2) protein in mammalian cells. Methods The yeast strain AH109 was transformed with AngRem104pGBKT7/c-myc and yeast-mating was utilized to screen for interacting proteins with AngRem104 in pretransformed human kidney cDNA library. The human embryonic kidney (HEK 293T) cells were transformed with two recombined plasmids,AngRem104-pcDNA3.1/V5-His and BBS2-pCMV/c-myc. Mouse anti-human V5 monoclonal antibody and mouse anti-human c-myc monoclonal antibody were used in immunoprecipitation and immunoblot analysis, respectively. Results Seven proteins that interact with AngRem104, including BBS2 were identified. The AngRem104-V5 and the BBS2-c-myc fusion protein were detected respectively in the immunoprecipitation by anti-c-myc and anti-V5 antibody. Conclusion The novel gene AngRem104 may interact with BBS2 protein in mammalian cells,which provides insights as to the function exploration of novel gene AngRem104 and the pathogenesis investigation of Bardet-Biedl syndrome.
