Effects of hypoxia on repair of rat small intestine epithelium cell damaged by ?- irradiation
- VernacularTitle:低氧对大鼠小肠上皮细胞辐射损伤修复的影响
- Author:
Fuyou WANG
;
Jingsheng GAO
- Publication Type:Journal Article
- Keywords:
irradiation;
hypoxia;
DNA repair;
cell cycle
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of hypoxia on repair of radiation damage and the possible mechanism. Methods Rat jejunal epithelium cell was taken as study object and divided into control group, irradiation group and irradiation-hypoxia group. The irradiation group was irradiated only once by 60 Co-? ray at the dose of 12 Gy and the irradiation-hypoxia group was treated with hypoxia (5% oxygen) for 6 h after irradiation. MTT was used to detect the survival rate of the irradiated cells, 3H-TdR incorporation was used to detect cell proliferation, and unscheduled DNA synthesis and single cell gel electrophoresis were used to detect DNA repair and flow cytometry was used to detect cell cycle. Results As compared with the irradiation group, the cells in the irradiation-hypoxia group repaired rapidly, proliferated obviously and the survival rate was increased about 10% (P
