Effects of adenosine A1 receptor-induced delayed preconditioning on heart preservation and its relation with NF-?B
- VernacularTitle:腺苷受体激动剂预处理对保存大鼠心脏的影响及其与NF-?B的关系
- Author:
Junyao LI
;
Ying TANG
- Publication Type:Journal Article
- Keywords:
adenosine;
preconditioning;
heart preservation;
nuclear factor-?B
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the potential improvement of donor heart storage by A1 adenosine receptor-induced delayed preconditioning and its relation with NF-?B. Methods A total of 64 male Wistar rats were randomly divided into 6 groups. Group A was pretreated with 2-chloro-N6-cyclopen-tyladenosine (CCPA), and Group B was only injected saline. Twenty-four hours later, the hearts in groups A and B were stored in St.Thomas solution for 4 h at 4 ℃ and reperfused with K-H buffer for 1 h. Group C was administered with CCPA after 15-minute saline injection, and Group D was injected with inhibitor of nuclear factor ?B, pyrrolidindiethyldithiocarbamate (PDTC) first and 15 min later injected with CCPA. Group E was only injected with saline and Group F with PDTC. Twenty-four hours later, the rat hearts were isolated, perfused with H-K buffer solution on the Langendorff apparatus, and subjected to hypothermic ischemia for 180 min with intermittent perfusion of modified St. Thomas solution, and reperfused with 37 ℃ H-K buffer for 60 min. Left ventricular function, myocardial CK-MB leakage, tissue levels of adenosine triphosphate were measured. Results The recovery rate of ?dp/dt max left ventricle in Group A were much higher than that of Group B, while that in Group C were much higher than that in groups D, E and F (P
