Increased expression of 5-hydroxytryptamine transporter gene in monocrotaline-induced pulmonary hypertension in rats
- VernacularTitle:野百合碱诱导的肺动脉高压大鼠肺血管5-HT转载体表达增强
- Author:
Chunguang YANG
;
Xinhua ZHANG
;
Mi PEI
;
Huailiang WANG
- Publication Type:Journal Article
- Keywords:
monocrotaline;
serotonin/5-hydroxytrapta-mine;
5-hydroxytraptamine transporter;
pulmonary hypertension
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the relationship between pul-monary hypertension (PHT) and 5-hydroxytrapta-mine transporter (5-HTT) in the pulmonary arteries (PAs) from normal and monocrotaline (MCT) induced pulmonary hypertension rats. Methods MCT-treated rats were used as a model for chronic PHT.Concentration-response curves of 5-hydroxytraptamine induced pulmonary vasoconstriction were established. The medial wall thickness of pulmonary arteries was measured. Semi-quantitative RT-PCR was performed to identify the expression of 5-HTT mRNA in rat PAs.Results 5-HT induced vasoconstriction response of PAs from MCT rats was significantly increased.The thickness of pulmonary vascular medial walls was increased in MCT rats. A significantly higher level of 5-HTT mRNA expression was detected in PAs from MCT rats. The ratio of the PCR products of 5-HTT gene to those of ?-actin gene was higher in MCT rats than in control rats. 5-HTT mRNA expression of pulmonary arteries correlated with the thickness of pulmonary vascular medial walls in rats.Conclusions MCT-induced pulmonary vascular remodeling and increased vascular contractile response to 5-HT were accompanied with enhanced level of 5-HTT mRNA expression and there existed correlation between the wall thickness of pulmonary arteries and 5-HTT mRNA expression,indicating 5-HTT is an important mechanism involved in pulmonary hypertension.
