Effect on expression of caspase-3 in the apoptosis of cultured humanumbilical vein endothelial cells induced by proteasome inhibitor MG132
- VernacularTitle:MG132诱导人血管内皮细胞凋亡及对caspase-3表达的影响
- Author:
Fang GUO
;
Jinsong TANG
;
Wenqing SUN
;
Lei WANG
;
Xiangdong YANG
- Publication Type:Journal Article
- Keywords:
ubiquitin-proteasome pathway;
protesome inhibitor;
vein endothelial cells;
apoptosis;
caspase-3
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of proteasome inhibitor MG132 on the expression of caspase-3 and apoptosis in cultured human umbilical vein endothelial cells.Methods Human umbilical vein endothelial cells was treated with MG132 (2,5 ?mol?L~(-1)) for 24 h. The apoptotic cells were determined by DNA fragment analysis and flow cytometric analysis. The level of caspase-3 mRNA was quantified by reverse transcription-polymerase chain reaction (RT-PCR). The protein contents of caspase-3 were analyzed by immunocytochemistry.Results The results showed that the increase of the degree of human umbilical vein endothelial cells apoptosis was concentration dependent. MG132 could up-regulate the gene/protein expression of caspase-3.Conclusions The results implicated that proteasome inhibitor MG132 induced human umbilical vein endothelial cells apoptosis by accumulation of caspase-3.
