Protective effects of indole-selenium on immunological liver injury in mice via inhibiting nitric oxide and proinflammatory cytokines production
- VernacularTitle:吲哚硒对免疫性肝损伤小鼠一氧化氮和炎性细胞因子的影响
- Author:
Ling JIANG
;
Hua WANG
;
Chengyi WU
;
Shengyi ZHANG
;
Wei WEI
- Publication Type:Journal Article
- Keywords:
indole-selenium;
melatonin;
selenium;
immunologic liver injury;
mice
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of indole-selenium on mice's immunological liver injury.Methods The model of immunological liver injury in mice was prepared. The activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were assayed by spectrophotometry; the levels of nitric oxide(NO) in serum were assayed by Griess method; tumour necrosis factor-? (TNF-?) activity was determined by ridio immunoassay method and the level of interleukin-1(IL-1) was assayed by MTT dye reduction. Results Indole-selenium (5, 10, 20 mg?kg~(-1)) significantly decreased serum transaminase (ALT, AST) levels,attenuated the area and extent of necrosis and reduced the infiltration of inflammatory cells. Furthermore, indole-selenium significantly lowered the increased TNF-? and NO levels in serum and inhibited the production of TNF-? and IL-1 by peritoneal macrophages. Conclusion Indole-selenium possesses amelioration effect on pathology of immunological liver injury in mice,which was related directly with inhibiting the production of TNF-? and NO levels in serum, decreasing the production of TNF-? and IL-1 by peritoneal macrophages.
