Establishing a rapid method to quantitatively measurethe bacterial endotoxin and fungus content in plasma of infectious patients
- VernacularTitle:体液内毒素及深部真菌快速定量检测方法
- Author:
Baojun YU
;
Yousheng LI
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
Endotoxin;
Fungus;
Infection;
Detection
- From:
Parenteral & Enteral Nutrition
1997;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Using MB-80 Microbiology Kinetic Rapid Reader, the methods were established to quantitatively measure the body fluid content of bacterial endotoxin and fungus in infectious patients. Methods: The blood samples were collected from Gram negative bacterial infection and deep mycosis patients and healthy volunteers. After pre-treatment, the plasma samples were measured using MB-80 system through kinetic turbidimetric limulus test method. The system computer could calculate the content of endotoxin and fungus during 1 hour detection. Results: The detection methods of endotoxin and fungus using MB-80 were simple and rapid. The process of pre-treatment was standardized and the results had good repeatability. The normal ranges of plasma endotoxin and fungus of human healthy volunteers were (2.03?2.32)ng/L and (2.46?2.15)ng/L respectively. The plasma endotoxin level of abdominal infection patients was increased significantly to 50-3 958 ng/L. The fungus level of deep mycosis patients was enhanced to 20-2 381 ng/L. Conclusion: The methods of endotoxin and fungus quantitatively measurement by MB-80 are simple and convenient and have results with good repeatability. The new detection methods of endotoxin and fungus can be used for diagnosis of endotoxemia and deep mycosis in early stage of infection.
