Irradiation Effect On The Apoptosis Induction In The Human Cancer Cell Lines And The Gingival Fibroblast.
- Author:
Moo Soon PARK
1
;
Sam Sun LEE
;
Soon Chul CHOI
;
Tae Won PARK
;
Dong Soo YOU
Author Information
1. Department of Oral & Maxillofacial Radiology, College of Dentistry, Seoul National University , Korea.
- Publication Type:Original Article
- MeSH:
Apoptosis*;
Cell Line*;
DNA;
Fibroblasts*;
Flow Cytometry;
Humans*;
KB Cells;
Propidium
- From:Journal of Korean Academy of Oral and Maxillofacial Radiology
1998;28(1):59-72
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The radiation-induced apoptosis was studied for two human cancer cell lines (KB cells, RPMI 2650 cells) and the human gingival fibroblast cell line (HGF-1 cells). The single irradiation of 2, 10, 20Gy was done with 241.5 cGy/min dose rate using the 137Cs MK cell irradiator. The cell were stained with propidium iodide and examined under the fluoro-microscope and assayed with the flow cytometry a day after irradiation. Also, the LDH assay was done to determine the amount of necrotic cells. The obtained results were as follows : 1. On the fluoro-microscope, many fragmented nuclei were detected in the KB, RPMI 2650, and HGF-1 cells after irradiation. 2. On the DNA content histogram obtained from the flow cytometry, the percentages of the pre-G1 peak of the control and 2, 10 and 20Gy irradiation group were 4.5, 55.0, 52.3, and 66.6% on KB cells, 2.7, 3.3, 31.8, and 32.6% on RPMI 2650 cells and 2.8, 21.8, 30.4, and 40.2% on HGF-1 cells respectively. 3. The number of G1-stage cells was abruptly decreased after 2Gy irradiation on KB cells and 10Gy irradiation on RPMI 2650 cells, But there was a slight decrease without regard to irradiation dose on HGF-1 cells. 4. There was no significantly different absorbance in extracellular LDH assay along the experimental cell lines.
