Proteome analysis of nuclear matrix proteins during arsenic trioxide induced apoptosis in K562 cells
- VernacularTitle:运用蛋白质组学研究三氧化二砷诱导K562细胞凋亡过程中核基质蛋白的变化
- Author:
Zihui WANG
;
Ding YU
;
Jie ZHENG
;
Ya CHEN
- Publication Type:Journal Article
- Keywords:
K562 cells;
Arsenic trioxide;
Nuclear matrix-associated proteins;
Apoptosis
- From:
Journal of Peking University(Health Sciences)
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate arsenic trioxide (As 2O 3) -target interactions at the level of nuclear matrix (NM) in chronic myelogenous leukemia cell line K562 by proteomics. Methods: DNA fragmentation analysis was used for As 2O 3 induced apoptosis of K562 cells. The nuclear matrix proteins were analyzed by high-resolution two-dimensional gel electrophoresis and computer-assisted image analysis. Results: While more than 200 protein spots were shared among the nuclear matrices, about 18 distinct spots were found characteristic of As 2O 3 treated cells. Onset of mass mange apoptosis, and the profiling of nuclear matrix proteins had been alternated and it was a more sensitive indicator than nucleosomal DNA fragmentation against As 2O 3 treatment. Conclusion: As 2O 3 induced apoptosis in K562 cells in a dose-time-dependent manner. As 2O 3 might be clinically useful in treatment of chronic myelogenous leukemia and the changes of nuclear matrix proteins in the treated cells can be used as a useful indicator for the treatment.
