In vitro amplification of immortalized keratinocytes with micro-carriers in rotary cell culture system
- VernacularTitle:永生化表皮细胞体外快速扩增的实验研究
- Author:
Yan RAO
;
Mingbin DAN
;
Deru LI
;
Zeqian FANG
- Publication Type:Journal Article
- Keywords:
immortalization;
skin tissue engineering;
rotary cell culture system;
microcarrier
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the way and technique to gain adequate seed cells for skin tissue engineering sufficiently. Methods Human telomerase reverse transcriptase (hTERT) gene was introduced into rabbit keratinocytes by eukaryotic vector. The positive clones were selected and cultured in microcarrier-RCCS. The growth of immortalized keratinocytes was observed, and the metabolic rate and pan-cytokeratins (AE1/AE3) expression of immortalized keratinocytes in experimental groups were detected and compared with those in the control group. Results The immortalized keratinocytes in the experimental groups grew rapidly and had a high metabolic rate and shorter population doubling time (PD) (P
