Multiple types of hematopoietic growth factors secreted spontaneously by human umbilical cord blood stromal cells in vitro
- VernacularTitle:人脐血基质细胞自发分泌多种造血生长因子的实验研究
- Author:
Yao LIU
;
Xinghua CHEN
;
Xi ZHANG
;
Xiangui PENG
- Publication Type:Journal Article
- Keywords:
human umbilical cord blood stromal cell;
hematopoietic growth factor;
bone marrow stromal cell
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential of stromal cells from human umbilical cord blood cultured in vitro to secrete hematopoietic growth factors. Methods The expressions of hematopoietic growth factors of stromal cells from human umbilical cord blood and bone marrow were detected by ELISA kits at different time points. Results Stromal cells from human umbilical cord blood could secrete thrombopoietin (TPO), granulocyte-macrophage colony stimulating factor (GM-CSF) and stem cell factor (SCF). The peak levels of TPO and SCF were found at 7 d after culture in vitro, but then the levels of these factors decreased gradually. Stromal cells from human umbilical cord blood secreted more SCF and GM-SCF, but less TPO than human bone marrow stromal cells. Conclusion Our results demonstrate that stromal cells from human umbilical cord blood could sustain hematopoiesis by secreting multiple types of hematopoietic growth factors such as TPO, GM-CSF, and SCF.
