Relationship between HVRⅠ mutation of mitochondrial DNA and lung squamous carcinoma
- VernacularTitle:线粒体DNA HVR区Ⅰ序列突变与肺鳞癌关系的研究
- Author:
Lihong LIU
;
Yide HU
- Publication Type:Journal Article
- Keywords:
lung squamous carcinoma;
mitochondrial DNA;
D-loop region;
hypervariable regionⅠ;
mutation
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between hypervariable regionsⅠ(HVRⅠ) mutation of mitochondrial DNA (mtDNA) and lung squamous carcinoma and to explore its significance in carcinogenesis. Methods White blood cells and carcinoma tissues were obtained from 13 cases of lung squamous carcinoma patients and mtDNA were extracted by one step method. HVRⅠfragments were amplified by PCR. Mutations were determined by DNA sequencing. Results In 13 lung squamous carcinoma patients, 8 cases showed mutation in HVR Ⅰ, and 30 mutations were found in 25 different nucleotide sites, in which 17 were point mutations and 13 were insertions and deletions, including a 10-bp deletion in one patient. Conclusion These results suggest that the mutation rate of HVRⅠsequence in lung squamous carcinoma tissue was relatively high, and point mutation might play an important role in lung carcinogenesis.
