Therapeutic effect of Caspase-1 inhibitor in experimental severe acute pancreatitis
- VernacularTitle:Caspase-1抑制剂对实验性重症急性胰腺炎的治疗作用
- Author:
Renmin ZHU
;
Xiaohua ZHANG
;
Zhaoshen LI
- Publication Type:Journal Article
- Keywords:
severe acute pancreatitis;
Caspase-1;
Interleukin-1?;
Interleukin-18
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Caspase-1 inhibitor on severe acute pancreatitis (SAP) in experimental SD rat model. Methods A model of SAP was reproduced by retrograde injection of 5% sodium taurocholate into the biliary-pancreatic duct. Heathy control (HC) rats underwent identical surgical procedure and duct cannulation without the injection of sodium taurocholate. Forty-two SD rats were randomly divided into three groups: healthy controls (HC, n=6); SAP-S group (n=18); SAP-ICE-I group (n=18). In SAP-S group, rats received intraperitoneal injection of isotonic saline 2 hours after induction of acute pancreatitis, and saline injection was repeated after 12 hours. In SAP-ICE-I group, rats were given ICE inhibitor intraperitoneally 2 hours after induction of pancreatitis. As in SAP-S group, this was repeated 12 hours laten. Surviving rats were killed at certain time, and all samples were obtained for subsequent analysis. Also, twenty-four rats were randomly divided into two groups: SAP-S group and SAP-ICE-I group, and the 24-hour death rate after SAP induction was observed. Results The serum amylase levels were increased significantly in SAP-S group (P0.05). Caspase-1 inhibition abated the severity of pancreatic tissue damage, and the 24-hour death rate was lowered from 91.7% to 41.7%. Conclusions The expressions of Caspase-1 activated cytokines IL-1? and IL-18 play a pivotal role in the pathogenesis of SAP. Caspase-1 inhibition significantly abates the severity and the mortality in SAP.
