Effect of mast cell tryptase on PAR-2 and MCP-1 mRNA expression of human renal fibroblasts in culture
- VernacularTitle:肥大细胞类胰蛋白酶对人肾成纤维细胞MCP-1和PAR-2表达的影响
- Author:
Daihong WANG
;
Fahuan YUAN
;
Yaoquan ZHANG
- Publication Type:Journal Article
- Keywords:
fibroblast;
tryptase;
benzamidine hydrochloride hydrate;
monocyte chemoattractant protein-1;
Protease-activated receptor 2
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To further investigate the effect of mast cell tryptase on the pathogenesis of chronic renal fibrosis. Methods Renal interstitial fibroblasts were isolated from histologically normal renal tissue obtained from kidneys removed because of malignant renal tumor by collagenase disintegration, and cultured in vitro. The cultured fibroblasts were identified by cell shape and immunocytochemistry, and divided into groups for further experiments. The expression of monocyte chemoattractant protein-1 (MCP-1) and protease-activated receptor 2(PAR-2) mRNAs in in vitro cultured fibroblasts was assessed by means of semi-quantitive reverse transcriptase polymerase chain reaction (RT- PCR). Result Renal fibroblasts were successfully cultured. Mast cell tryptase(10~500ng/ml) promoted expression of PAR-2 mRNA and MCP-1 mRNA of human renal fibroblasts in heparin-dependent manner. Proteinase inhibitor benzamidine hydrochloride hydrate (200?mol/L) could suppress the effects of tryptase on the cultured renal fibroblasts. But TGF-? antibody did not influence the effect. Conclusion Tryptase might be involved in pathogenesis and development of renal fibrosis by PAR-2 and MCP-1.
