Protective efficiency and neurotransmitter mechanism of three methods of isoflurane intervention on various levels of global cerebral ischemic injury in rats
- VernacularTitle:异氟烷不同时间干预对大鼠不同程度全脑缺血的保护作用及其神经递质机制的探讨
- Author:
Yonghai SUN
;
Yun YUE
;
Yun WANG
- Publication Type:Journal Article
- Keywords:
preconditioning;
reperfusion injury;
excitatory amino acids;
microdialysis;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the neurotransmitter mechanism and protective efficiency of three methods of isoflurane intervention on global cerebral ischemia, and to seek an effective method to improve cerebral ischemia damage. Methods Adult male Sprague-Dawley rats were randomly divided into sham operation group, control groups, preconditioning groups, protective groups and resuscitation groups. The rats of the last four groups were further divided into 10, 15 and 20min ischemia subgroups. The model of global cerebral ischemia and reperfusion was reproduced in waking rats 2 days before ischemia. The microdialysis samples were collected and BIS was recorded after reperfusion. The recovery of right reflection was observed after ischemia and the motor function was observed. All viable and apoptotic neurons were counted and the percentage of apoptotic neurons was calculated. The results showed that glutamate concentration in the hippocampus of protective groups was significantly lower compared with preconditioning group and resuscitation group. Results With ischemia fasting 10 and 15min (P
