Angiotensin n induces apoptosis in tubular epithelial cells through inhibiting the phospho-rylation of serine-threonine kinase Akt/protein kinase B
- VernacularTitle:血管紧张素Ⅱ通过抑制AKT/蛋白激酶B磷酸化诱导肾小管上皮细胞凋亡
- Author:
Jili ZHU
;
Guohua DING
;
Xueyu WANG
- Publication Type:Journal Article
- Keywords:
Angiotensin II;
Apoptosis;
AKT/protein kinase B;
1-phosphatidylinositol 3-kinase;
Renal tubular epithelial cell
- From:
Chinese Journal of Nephrology
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the signaling pathway of PI3K-AKT is involved in Ang II-induced apoptosis in rat renal tubular epithelial cells (NRK-52E).Methods Cultured cells were incubated in media containing either buffer (control) or different concentrations of Ang II (10-9 to 10-6 mol/L) for 24 h. Cells were stained by Annexin V-F1TC/PI Kit and apoptosis was evaluated by flow cytometer. Western blotting was performed to assess the levels of PI3K, total- and phosphor-AKT. Results Ang II induced tubular epithelial cell apoplosis in a dose dependent manner. Ang II significantly inhibited the phosphorylation of AKT. The level of AKT phosphorylation was negatively correlated with apoptosis in Ang II-stimulated cells(r=-0.90 ,P
