Effects of mimic ischemic preconditioning on [Ca~(2+)]_i and L-type calcium current of cultured sinoatrial node cells of neonatal rat
- VernacularTitle:模拟缺血预适应对原代培养乳鼠窦房结细胞内钙及L-型钙电流的影响
- Author:
Qian ZHANG
;
Zhiyuan SONG
;
Shifei TONG
;
Ji HUANG
- Publication Type:Journal Article
- Keywords:
sinoatrial node cell;
ischemic preconditioning;
calcium ion;
L-type calcium current
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of mimic ischemic preconditioning (IP) on i and I-LCa of sinoatrial node cells and explore the protective effects of IP. Methods Cells were randomized to three groups: control, ischemia/reperfusion (I/R), IP. After labeled with fura4, fluorescence intensity of i was studied with laser confocal microscopy and I-LCa was recorded by whole-cell patch clamp technology. Results IP significantly reduced the fluorescence intensity of i and enhanced the peak of I-LCa as compared with I/R, shifted the I-V curve to more negative value. Conclusion IP can reduce the overload of i caused by I/R and increase I-LCa weakened by I/R.
