Effects of up-regulation of separase/esp1 on tumor chromatid separation and construction of a conditional expression vector
- VernacularTitle:Separase上调对肿瘤细胞染色体分离作用的研究及条件表达载体的构建
- Author:
Liyue YOU
;
Xin LIU
- Publication Type:Journal Article
- Keywords:
spc cell;
esp1;
transfection;
tetracycline
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct an expression vector containing esp1 in order to investigate the effects on the chromatid separation. Methods A conditional plasmid vector containing esp1 was constructed, and was transfected into spc cells. The expression level of esp1 was detected by fluorescence microscopy, and the chromosomes were observed. Results EGFP-N1-esp1 was expressed in spc cells, and improper chromosome segregation was reduced. The plasmid of pTRE-d2EGFP-esp1 was constructed successfully. Conclusion Up-regulation of esp1 has inhibitory effects on the abnormal chromatid separation of tumors and may be potential in the treatment of cancer.
