Effects of sense and antisense T-STAR gene on colon cancer cell line HCT-116
- VernacularTitle:T-STAR基因对人结肠癌细胞系HCT-116生长增殖的影响
- Author:
Ling ZHANG
;
Lian GUO
;
Yong PENG
;
Bin CHEN
- Publication Type:Journal Article
- Keywords:
testes-signal transduction and activator of RNA;
antisense nucleic acid;
sense nucleic acid;
cell transfection
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of the sense and antisense testes-signal transduction and activator of RNA (T-STAR) gene on the colon cancer cell line HCT-116. Methods The sense and antisense T-STAR gene was stably transfected into HCT-116 cells with lipofectamine. The expression level of T-STAR in those cells was detected by Western blotting and the growth velocity and proliferation of those cells by cytokinetics. Results The growth velocity and proliferation decreased after transfection of the sense T-STAR gene, but increased after transfection of the antisense T-STAR gene. Conclusion T-STAR gene can inhibit the growth velocity and proliferation of HCT-116 cells.
