Prokaryotic expression, purification and identification of anti-bacterial peptide gloverin
- VernacularTitle:抗菌多肽葛佬素的原核表达纯化及活性鉴定
- Author:
Bo XU
;
Hong ZHOU
;
Huizeng LI
;
Yongling LU
;
Liangxi WANG
;
Guof DING
- Publication Type:Journal Article
- Keywords:
gloverin;
anti-bacterial peptide;
prokaryotic expression;
lipopolysaccharide
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express anti-bacterial peptide gloverin in prokaryotic system and investigate its bioactivity. Methods E.coli BL21 was used to express gloverin. Western blot was used to identify the production and Tachypleus Amebocyte Lysate (TAL) was used to detect the bioactivity of the production. Results The production was identified as gloverin peptide by Western blotting, and the TAL data indicated that the production could neutralize LPS. Conclusion Anti-bacterial peptide gloverin is successfully expressed in E.coli BL21.
