Magnolol induces apoptosis via alteration of Bax/Bcl-X_L ratio and activation of caspases
- VernacularTitle:厚朴酚通过改变Bax/Bcl-X_L表达和激活caspase诱导人宫颈癌HeLa细胞凋亡
- Author:
Mingyu XIA
;
Minwei WANG
;
Shinichi TASHIRO
;
Takashi IKEJIMA
- Publication Type:Journal Article
- Keywords:
magnolol;
HeLa cell;
apoptosis
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the mechanism of magnolol-induced HeLa cell death.Methods Cell viability was measured by MTT method.Morphological changes were observed by phase contrast microscopy and Hoechst 33258 staining. DNA fragmentation was assayed by agarose gel electrophoresis. Protein level was detected by Western blot analysis.Results Magnolol induced HeLa cell apoptosis and had a weaker cytotoxic effect on HEL 299 cell. The apoptosis of HeLa cells was partially reversed by caspase-3,-8,-9,-10 and caspase family inhibitors. Magnolol has a synergistic apoptotic effect with Fas agonistic antibody CH-11. Treatment of HeLa cells with magnolol for 12 h increased the protein expression ratio of Bax/Bcl-X_L; procaspase-3, ICAD and PARP were cleaved to smaller molecules. p53 and phosphorylation of p53 (ser-15) increased response to magnolol treatment.Conclusion Magnolol induced HeLa cell death via alteration of Bax/Bcl-X_L ratio,activation of caspases and accumulation of p53.
