Problems in detecting CK19 mRNA with RT-PCR and corresponding countermeasures
- VernacularTitle:RT-PCR检测外周血中CK19 mRNA方法的优化
- Author:
Jianwei BI
;
Wei JING
;
Jun ZHANG
;
Wenji LI
- Publication Type:Journal Article
- Keywords:
stomach neoplasms;
cytokeratin19;
micrometastasis;
RT-PCR
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To search for a better RT-PCR method for detecting CK19 mRNA. Methods: RT-PCR methods were used to detect the expression of CK19 mRNA in the tumor tissues and in the peripheral blood of patients with gastric cancer and in the peripheral blood of healthy subjects. Results: The influence of pseudogene and genome was observed through nonreversely transcripted RNA controls and comparison of several pairs of primers.The interference could be effectively eliminated by regulating the annealing temperature based on mismatched primers. Conclusion: The presence of pseudogene and genome interferes the experimental protocol in RT-PCR,which can be resolved with the method introduced in our paper.
