Effects of shenfu injection on nuclear factor-?B during myocardial ischemia/reperfusion injury in rats
- VernacularTitle:参附注射液对大鼠急性缺血/再灌注损伤心肌核转录因子-?B的影响
- Author:
Benjing ZHANG
;
Yanlin WANG
;
Chengyao WANG
- Publication Type:Journal Article
- Keywords:
shenfu injection;
ischemia;
reperfusion;
nuclear factor-kappa B;
tumor necrosis factor-alpha;
interleukin-6
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the protective effects of shenfu injection on myocardial ischemia/reperfusion (I/R) injury in rats and its potential mechanisms. METHODS: Myocardial ischemia/reperfusion was produced by tying and untying of left anterior descending coronary artery. Ischemia lasted for 30 min and reperfusion for 60 min. Twenty-four healthy male SD rats weighing 230-280 g were randomly divided into three groups (n=8 in each): sham-operation group, I/R group, and shenfu group which the shenfu injection (10 ml?kg -1) were injected intraperitoneally 30 min before ischemia. The plasma concentration of tumor necrosis factor-? (TNF-?), interleukin-6(IL-6) were measured by ELISA. The heart was harvested and levels of the nuclear factor kappaB (NF-?B) activity were determined by Ecl-western blot analysis and ultrastructures were observed by electron microscopy. RESULTS: NF-?B binding activity in myocardial nuclear and the plasma concentration of IL-6, TNF-? were significantly increased in I/R group than that in the sham-operation group (P
