Regulatory Action of BushenqingreFang on Apoptosis of Nephridial Tissue in Mice with Lupus Nephritis Caused by cGVHD
- VernacularTitle:补肾清热毒方对cGVHD狼疮小鼠肾组织细胞凋亡的调节作用
- Author:
Yangrong CHEN
;
Wenying REN
;
Ming JIANG
- Publication Type:Journal Article
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the regulatory action of BushenqingreFang on Fas/FasL in the cells of nephridial tissue in mice with lupus nephritis (LN) caused by chronic graft-versus-host disease (cGVHD). Methods: To observe the apoptosis in nephridial tissue by TUNEL and to measure the Fas/FasL genetic transcription and their protein expression by immunohistochemistry, western blot and RT-PCR techniques. Results: BushenqingreFang increased the positive score of TUNEL in nephridial tissue and up regulated mRNA expression and protein levels of Fas and FasL. Conclusion: The action of BushenqingreFang on LN may be conducted by its up regulation of Fas and FasL and its promotion of apoptosis.
