Breeding, reproducing, and identifying SRC-3 knock-out mice
- VernacularTitle:SRC-3基因敲除小鼠的饲养繁殖及鉴定
- Author:
Zhiyong DU
;
Yongping SU
- Publication Type:Journal Article
- Keywords:
steroid receptor coactivator-3;
gene knock out;
mice
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To breed and identify steroid receptor coactivator-3 (SRC-3) knock-out mice. Methods Heterozygote mice were bred and reproduced. Wild genotype, heterozygote genotype, and homozygote genotype would appear in offsprings of parents. Genome DNA extracted from the murine tails was subjected to PCR for genotype identification. Male homozygote mice were selected to mate with the female heterozygote mice for acquiring homozygote baby mice according to Mendel law. Results Breeding and reproducing were successful and more heterozygote genotype mice were reproduced. Conclusion Appropriate methods for breeding, reproducing, and identifying are the effective way for acquiring SRC-3 knock-out mice from heterozygote mice.
