Latency of herpes simplex virus 1 in the ciliary body of normal human eyes
- VernacularTitle:单纯疱疹病毒Ⅰ型在正常人眼睫状体潜伏的初步研究
- Author:
Kang CHEN
;
Hanping XIE
- Publication Type:Journal Article
- Keywords:
herpes simplex virus type 1;
latency;
ciliary body
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether there is latency of herpes simplex virus 1 (HSV-1) in the ciliary body of normal human eyes. Methods Parts (1/3) of the 15 fresh eyeballs from normal healthy donors were used for immunohistochemical staining to exclude from acute infection of HSV-1. After the residual ciliary bodies were taken out, 1/3 was centrifugated for co-culture with Vero cells, and DNA was extracted from the other 2/3 for PCR and gel electrophoresis for the detection of HSV-1 DNA and latency associated transcript (LAT). Results All the 15 samples were negative of immunohistochemical staining and the results of co-culture of Vero cells with the supernatant containing the ciliary body tissues were also negative. No pathological changes in the cells were observed. In the 15 samples, no. 4, 5, 7 samples were positive of HSV-1 DNA but negative of HSV-1 LAT, whereas the other 12 samples were negative of HSV-1 DNA and HSV-1 LAT. Conclusion HSV-1 DNA exists in the normal human cilicary body.
